Li-Lin Du, Ph.D. - Postgraduate - 北京生命科学研究所

Li-Lin Du, Ph.D.

Investigator, NIBS, Beijing, China

Phone：010-80726688-8505

Fax： 010-80726689

E-mail：dulilin@nibs.ac.cn

Education

1992	B.Sc., Department of Biology, Nankai University, China
1995	M.Sc., Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China
2001	Ph.D., Department of Molecular Biophysics & Biochemistry (MB&B), Yale University, USA

Professional Experience

2001-2007	Postdoctoral Research Associate in Dr. Paul Russell's lab at The Scripps Research Institute (TSRI), USA
2007-2013	Assistant Investigator, National Institute of Biological Sciences, Beijing, China
2013-2021	Associate Investigator, National Institute of Biological Sciences, Beijing, China
2021.6	Investigator, National Institute of Biological Sciences, Beijing, China

Research Description:

We use the fission yeast Schizosaccharomyces pombe as a model system. Working with fission yeast has many advantages, including the ease of genetics and cell biology, availability of powerful functional genomics tools, and extensive homology between its genes and human genes.

We are carrying out research on the following topics:

1. Studying the mechanisms of DNA double strand break repair.

2. Dissecting the molecular machineries of autophagy and related trafficking pathways.

3. Systematic screening of genetic interactions.

Publications:

1. Sun L-L, Li M, Suo F, Liu X-M, Shen E-Z, Yang B, Dong M-Q, He W-Z, and Du L-L. Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9: e1003715 (2013).

2. Yu Y, Ren J-Y, Zhang J-M, Suo F, Fang X-F, Wu F, and Du L-L. A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks. DNA Repair 12:433-443 (2013).

3. Zhou Z-X, Zhang M-J, Peng X, Takayama Y, Xu X-Y, Huang L-Z, and Du L-L. Mapping genomic hotspots of DNA damage by a single-strand-DNA-compatible and strand-specific ChIP-seq method. Genome Res. 23: 705-715 (2013).

4. Qu M, Yang B, Tao L, Yates JR 3rd, Russell P, Dong M-Q, and Du L-L. Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint. PLoS Genet. 8: e1002817 (2012).

5. Li P, Li J, Li M, Dou K, Zhang M-J, Suo F, and Du L-L. Multiple end joining mechanisms repair a chromosomal DNA break in fission yeast. DNA Repair 11: 120–130 (2012).

6. Li J, Zhang J-M, Li X, Suo F, Zhang M-J, Hou W, Han J, and Du L-L. A piggyBactransposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 39: e40 (2011).

7. Sofueva S, Du L-L, Limbo O, Williams JS, and Russell P. BRCT domain interactions with phospho-histone H2A target Crb2 to chromatin at double-strand breaks and maintain the DNA damage checkpoint. Mol. Cell. Biol. 30: 4732–4743 (2010).

8. Liu N-N, Han TX, Du L-L, and Zhou J-Q. A genome-wide screen forSchizosaccharomyces pombe deletion mutants that affect telomere length. Cell Res. 20: 963–965 (2010).

9. Han TX, Xu X-Y, Zhang M-J, Peng X, and Du L-L. Global fitness profiling of fission yeast deletion strains by barcode sequencing. Genome Biol. 11: R60 (2010).

10. Martín V, Du L-L, Rozenzhak S, and Russell P. Protection of telomeres by a conserved Stn1-Ten1 complex. Proc. Natl. Acad. Sci. U.S.A. 104: 14038–14043 (2007).

11. Du L-L, Nakamura TM, and Russell P. Histone modification-dependent and -independent pathways for recruitment of checkpoint protein Crb2 to double-strand breaks. Genes Dev. 20: 1583–1596 (2006).

12. Coulon S, Noguchi E, Noguchi C, Du L-L, Nakamura TM, and Russell P. Rad22^Rad52-dependent repair of ribosomal DNA repeats cleaved by Slx1-Slx4 endonuclease. Mol. Biol. Cell. 17: 2081–2090 (2006).

13. Nakamura TM, Moser BA, Du L-L, and Russell P. Cooperative control of Crb2 by ATM family and Cdc2 kinases is essential for the DNA damage checkpoint in fission yeast. Mol. Cell. Biol. 25: 10721–10730 (2005).

14. Du L-L, Moser BA, and Russell P. Homo-oligomerization is the essential function of the tandem BRCT domains in the checkpoint protein Crb2. J. Biol. Chem. 279: 38409–38414 (2004).

15. Nakamura TM, Du L-L, Redon C, and Russell P. Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast. Mol. Cell. Biol. 24: 6215–6230 (2004).

16. Noguchi E, Noguchi C, Du L-L, and Russell P. Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1. Mol. Cell. Biol. 23: 7861–7874 (2003).

17. Du L-L, Nakamura TM, Moser BA, and Russell P. Retention but not recruitment of Crb2 at double-strand breaks requires Rad1 and Rad3 complexes. Mol. Cell. Biol. 23: 6150–6158 (2003).

18. Du L-L, and Novick P. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae. Mol. Biol. Cell. 13: 503–514 (2002).

19. Zhang X, Bi E, Novick P, Du L, Kozminski KG, Lipschutz JH, and Guo W. Cdc42 interacts with the exocyst and regulates polarized secretion. J. Biol. Chem. 276: 46745–46750 (2001).

20. Du LL, and Novick P. Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p. Mol. Biol. Cell.12: 1215–1226 (2001).

21. Du LL, and Novick P. Purification and properties of a GTPase-activating protein for yeast Rab GTPases. Meth. Enzymol. 329: 91–99 (2001).

22. Zhao F, Xu S, Du L, and Xu G. AMP makes native snake muscle fructose-1, 6-bisphosphatase to an alkaline enzyme. Sci. China, C, Life Sci. 43: 1–7 (2000).

23. Du LL, Collins RN, and Novick PJ. Identification of a Sec4p GTPase-activating protein (GAP) as a novel member of a Rab GAP family. J. Biol. Chem. 273: 3253–3256 (1998).